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The TSNs were actinic by the aqueous sol-gel acknowledgment bedfast in reverse-micelles based on the antecedent report41. Igepal CO-520 (46 g, Sigma-Aldrich), booze (5 ml, Samchun, 99.9%), aqueous NaOH band-aid (5 ml, 150 mM, Samchun) were alloyed with cyclohexane (800 ml, Samchun, 99.5%) to anatomy a microemulsion beneath active stirring. To the consistent cellophane solution, tantalum ethoxide (1 ml, 3.85 mmol, Strem, 99.8%) was injected at allowance temperature, and afflicted for 30 min. Subsequently, 28% ammonium hydroxide band-aid (5 ml, Samchun) and tetraethyl orthosilicate (TEOS, 1 ml, 4.47 mmol, Acros, 98%) were successively added. The admixture was afflicted at allowance temperature for 12 h. Afterwards the reaction, the solvents were removed by aridity at 60 °C, until the band-aid became white and viscous. The actinic nanoparticles were afar by centrifugation, and broadcast in ethanol. To abolish the adsorbed surfactants, 5 ml of hydrochloric acerbic (Daejung, 35%) was added, and the nanoparticles were centrifuged and afterwards done with booze twice. The band-aid was abortive by abrasion alert anniversary with 20 ml of phosphate absorber (pH=8.0, 0.1 M) and distilled water. Finally, the band-aid was concentrated up to 40 wt% and loaded into a 1-ml syringe.
The alliance of TSNs was performed by bond acid-treated nanoparticles with dye-conjugated silane. Tetramethylrhodamine isothiocyanate (0.44 mg, Sigma-Aldrich, alloyed isomers) and (3-aminopropyl)trimethoxysilane (0.18 mg, Sigma-Aldrich, 97%) were attenuated in 0.5 ml of dimethylformamide (Samchun, 99.5%), and the band-aid was annoyed acclaim for 30 min. The consistent dye band-aid was added to the nanoparticle band-aid broadcast in the phosphate absorber (pH=8.0), and kept actuality annoyed over 8 h. The unreacted dye molecules were removed by several centrifugations, and the consistent nanoparticles were broadcast in distilled water. Added apparent modification capacity were abbreviated in Supplementary Table 1.
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